(ARE-mRNA database) contains the complete entries of human ARE-containing
full-length mRNAs (see methodology for details).
ARED is further clustered into five groups depending on the number of
motifs in the ARE stretch. Groups 1-4 contain five, four, three and two
pentameric (AUUUA) repeats, respectively, while Group 5 contains only one
repeat within the 13-bp pattern. Clustering was performed in such a
way that, for example, Group 1 included not only exact five or more
continuous ARE pentamers but also those with 10% ambiguity, so that a
stretch of NUUUAUUUAUUUAUUUAUUUN would fall in this category. This process
was verified by a phylogenic tree relationship using Clustal-W alignment
of ARE stretches and their variations. As could be expected, this analysis
showed that the lower the number of ARE motifs in a group, the higher the
number of sequences that were included, and apparently the more
functionally diverse the corresponding ARE-genes.